Anion exchange resins in phosphate form as versatile carriers for the reactions catalyzed by nucleoside phosphorylases

• Julia N. Artsemyeva,
• Ekaterina A. Remeeva,
• Tatiana N. Buravskaya,
• Irina D. Konstantinova,
• Roman S. Esipov,
• Anatoly I. Miroshnikov,
• Natalia M. Litvinko and
• Igor A. Mikhailopulo

Beilstein J. Org. Chem. 2020, 16, 2607–2622, doi:10.3762/bjoc.16.212

• were tested in the synthesis of nelarabine, kinetin riboside, and cladribine with good to excellent yields (52–93%). Keywords: anion exchange resins; N6-benzyladenosine; cladribine; enzymatic glycosylation; kinetin riboside; nelarabine; α-ᴅ-pentofuranose-1-phosphates; phosphorolysis of nucleosides

• into BAPR was only 3% (all data from HPLC analysis) (Table 1). The results show that, depending on the structure of the matrix, phosphate anion exchange resins can be used as a source of inorganic phosphate ion in the reaction of enzymatic phosphorolysis of nucleosides (e.g., Dowex-nPi) or to perform a

Enzymatic synthesis and phosphorolysis of 4(2)-thioxo- and 6(5)-azapyrimidine nucleosides by E. coli nucleoside phosphorylases

• Vladimir A. Stepchenko,
• Anatoly I. Miroshnikov,
• Frank Seela and
• Igor A. Mikhailopulo

Beilstein J. Org. Chem. 2016, 12, 2588–2601, doi:10.3762/bjoc.12.254

• h; HPLC analysis of the reaction mixtures see Experimental section and Supporting Information File 1. Phosphorolysis of nucleosides 1b–5b and related pyrimidine nucleosides (2’-deoxyuridine, thymidine, 2- and 4-thiothymidines) catalyzed by E. coli UP (Figure 1) and TP (Figure 2). Geometry optimized